Driving Forces of Translocation Through Bacterial Translocon SecYEG
نویسندگان
چکیده
منابع مشابه
Protein Translocation: The Sec61/SecYEG Translocon Caught in the Act
The Sec61/SecYEG complex mediates both the translocation of newly synthesized proteins across the membrane and the integration of transmembrane segments into the lipid bilayer. New cryo-electron microscopy studies show ribosome-channel complexes in action and reveal their repertoire of conformational states.
متن کاملThe structure of the bacterial protein translocation complex SecYEG.
Proteins destined for secretion, membrane insertion or organellar import contain signal sequences that direct them to the membrane. Once there, transport machines receive and translocate them appropriately across or into the membrane. The related SecY and Sec61 protein translocation complexes are ubiquitous components of machines that are essential for protein transport. They co-operate with va...
متن کاملMembrane protein insertion and proton-motive-force-dependent secretion through the bacterial holo-translocon SecYEG-SecDF-YajC-YidC.
The SecY/61 complex forms the protein-channel component of the ubiquitous protein secretion and membrane protein insertion apparatus. The bacterial version SecYEG interacts with the highly conserved YidC and SecDF-YajC subcomplex, which facilitates translocation into and across the membrane. Together, they form the holo-translocon (HTL), which we have successfully overexpressed and purified. In...
متن کاملMembrane protein insertion and assembly by the bacterial holo-translocon SecYEG–SecDF–YajC–YidC
Protein secretion and membrane insertion occur through the ubiquitous Sec machinery. In this system, insertion involves the targeting of translating ribosomes via the signal recognition particle and its cognate receptor to the SecY (bacteria and archaea)/Sec61 (eukaryotes) translocon. A common mechanism then guides nascent transmembrane helices (TMHs) through the Sec complex, mediated by associ...
متن کاملSupplementary Information Energy barriers and driving forces of tRNA translocation through the ribosome
Supplementary Note 1 (Methods) 18 1.1 General molecular dynamics setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 1.2 Models of the ribosome including tRNAs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18 1.3 Refinement of the atomic models against cryo-EM maps . . . . . . . . . . . . . . . . . . . . . 19 1.4 Choice of models for simulation. . . . . . . ...
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ژورنال
عنوان ژورنال: The Journal of Membrane Biology
سال: 2018
ISSN: 0022-2631,1432-1424
DOI: 10.1007/s00232-017-0012-9